Bacterial Alpha-Amylase TB-2 is produced from selected strains of Bacillus subtilis through submerged fermentation, extraction and refining process. It can rapidly decreases the viscosity of the gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-1, 4 glycosidic bonds and produces soluble dextrins and oligosaccharides.
The powerful amylase activity of TB-2 is capable to break down the starches contained in the textiles into soluble polysaccharides, thus makes it washed out easily.
TB-2 is active at pH ranges from 6.0 to 7.0 with an optimum pH at 6.0. It can be inactivated dramatically at pH below 5.0.
EFFECT OF TEMPERATURE
TB-2 is active at temperatures from 30 to 90ºC, with an optimum temperature range from 60 to 90ºC. At temperatures from 70 to 90ºC, the reaction speeds up with the temperature rises while the enzyme inactivation also speeds up.
EFFECT OF CALCIUM
Calcium ion plays a protective role for the stability of alpha-amylase activity for TB-2. Its activity may disappear completely without the presence of calcium ion.
Copper, titanium and cobalt ions are moderate inhibitors for TB-2, whereas aluminum, lead and zinc ions act as strong inhibitors.
APPLICATION AND DOSAGE REFERENCE
Alpha-Amylase TB-2 is suitable for desizing of textiles sized by starchy materials, particularly fits to those textiles that are not heat resistance, such as cotton, silk, synthetic fabrics. Use of TB-2 enables mild operational conditions, no pollution, no damage to the fabrics, easily to wash out the starchy materials.
Cotton, Silk, Synthetic fibers
1. Protection salts should be added (0.025% CaCl2, 0.4% NaCl2) prior to adding TB-2.
2. The dosage rate in the above-mentioned table is only for reference. The actual dosage should be determined after a numbers of trials according to actual conditions.