Alpha-amylase HA-360 (Thermostable, Low pH)

As a very robust Alpha-Amylase with superior thermal and pH stability, HA-360 is applied to the production of ethanol.


HA-360 is a Heat-stable Alpha-Amylase produced from strains of Bacillus licheniformis through submerged fermentation and extraction refining processes. It is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.


As a very robust Alpha-Amylase with superior thermal and pH stability, HA-360 is applied to the production of ethanol.



     Declared Enzyme:


     Systematic Name:

EC, 1,4-α-D-glucan glucanohydrolase


180,000 U/ml (minimum)


Sepia brown liquid

     Product pH:

5.5 to 7.0

     Specific gravity:

1.10 to 1.25 g/ml



HA-360 is active over a wide pH range, depending on the application. The effective pH range is 4.8~6.0. For maximum activity, the optimal pH for HA-360 is 5.0 to 5.9. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.



For interval liquefaction, the temperature range of HA-360 is 80 to 100°C, the optimum temperature range is 83 to 86°C. It liquefies starch substrates promptly at 90 to 95ºC and maintains highly active at 100ºC. For consecutive liquefaction, HA-360 demonstrates exceptional

thermo-stability at a temperature range of 103 to 107ºC, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.



The presence of calcium increases the thermal stability of HA-360. This enzyme requires a very low calcium concentration and calcium additions of 20 to 50 ppm (dry solids basis) are sufficient to ensure enzyme stability.



Copper, titanium, and cobalt ions are moderate inhibitors for HA-360. Aluminum, lead and zinc ions act as strong inhibitors.


In general, first adjust the pH to 5.4 to 6.0, then add HA-360. Calcium is not needed. After this enzyme is added, heat the slurry to liquefy at a temperature range of 83 to 86ºC for 90 to 150 minutes. The final DE value should be maintained at 10 to 12.

Dosage of HA-360 is dependent on the starch substrate, application, process, substrate concentration and other factors. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage rate is 0.16 to 0.20KG/TDS.


Standard packaging is 25kg, 30kg food-grade HDPE drums or 1125 kg IBC totes.

Typical shelf-life is six months if this product is stored below 25°C in its original packaging, sealed and unopened, protected from sunlight. Its shelf-life will be eighteen months if this product is stored properly at 0 ∼10°C. This product has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperature may lead to a higher dosage requirement, therefore should be avoided.


Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.

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Factory 1: 



Contact:      Edna Yang

Tel:              +86 510 8525 9383 



Factory 2:



+86 151 5221 3005

Jiangsu Boli Bioproducts Co., Ltd.

93 Yangzhou Rd, Jiangyan Economic Development Zone,Taizhou City,Jiangsu 225500, China  

Jiangsu Boyang Bioproducts Co., Ltd.

30 Tongwang Road, Nantong Economic & Technological Development Zone,Nantong City,Jiangsu 226017, China

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PO Box 31096 Saint Louis,MO 63131 

Contact:David Singer 



Office:314 991 5191 

Mobile:314 503 0556 

Contact:Song Qian 


Contact:Ms.Edna Yang

Tel:+86 510 8525 9383 

Fax: +86 510 8522 1582 

Mobile:+86 151 5221 3005 



Address:93 Yangzhou Road,Jiangyan Economic Development Zone,Taizhou City,Jiangsu Province,China.

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