Alpha-amylase HA-580LFG (Thermostable, Low pH)
Use of HA-580LFG in the liquefaction of starch materials enables lower dosage level, less storage  and handling costs for the production of alcohol.


HA-580LFG is a high concentrated food-grade alpha-amylase produced from Bacillus licheniformis through submerged fermentation and refining extraction processes. It is an endoamylase that can  hydrolyze starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to  produce soluble dextrins and oligosaccharides, thus decrease the viscosity of gelatinous starch  solutions rapidly. Use of HA-580LFG in the liquefaction of starch materials enables lower dosage level, less storage  and handling costs for the production of alcohol. 


Declared Enzyme:            Alpha-Amylase 

Systematic Name:            EC, 1,4-α-D-glucan glucanohydrolase 

Activity:                             400,000 U/ml (minimum) 

Appearance:                    Sepia brown liquid 

Product pH:                      5.0 to 7.0 S

pecific gravity:                  1.10 to 1.25 g/ml 


HA-580LFG can work at pH from 4.8 to 6.0, with its optimal pH range from 5.0 to 5.9. The exact pH  optimum depends on the actual processing conditions, including temperature, substrate  concentration and processing time. 


For interval liquefaction, HA-580LFG works at temperatures from 80 to 100°C with it optimum  temperature range from 83 to 86°C. HA-580LFG can liquefy starch substrates promptly at 90 to  95ºC and maintain highly active at 100ºC.  For consecutive liquefaction, HA-580LFG demonstrates its exceptional thermo-stability at a  temperature range of 105 to 110ºC and keeps on liquefying efficiently for 5 to 7 minutes. 


Copper, titanium, and cobalt ions are moderate inhibitors for HA-580LFG; aluminum, lead and zinc  ions are strong inhibitors for this enzyme. 


First adjust pH to 5.0 to 5.9, and then add HA-580LFG. Heat the slurry for liquefaction at a  temperature range from 83 to 86ºC for 90 to 150 minutes. Final DE value should be maintained at a  range from 10 to 12.  

Dosage of HA-580LFG depends on the actual conditions such as kind of starch, substrate concentration, processing time, temperature, pH, etc. To optimize the dosage, it is recommended  to conduct a number of liquefaction trials prior to routine use of this product. A good starting point  for dosage rate is 0.075 to 0.10KG/TDS.


  • HA-580LFG is packaged in 25kg, 30kg food-grade HDPE plastic drums or 1125kg IBC totes.  Customized packaging is available upon request. 

  • Typical shelf-life is six months if it is stored below 25°C in its original packaging, sealed and  unopened, protected from direct sunlight.  

  • Its shelf-life will be eighteen months if it is stored properly at 0∼10°C.  

  • This enzyme has been formulated for optimal stability. Prolonged storage and/or adverse  conditions such as higher temperatures may lead to a higher dosage requirement, therefore  should be avoided. 


Inhalation of enzyme dust and mists should be avoided. In case of contact with skin or eyes,  promptly rinse with water for at least 15 minutes. For detailed handling information, please  refer to the Safety Data Sheet.



Factory 1: 



Contact:      Edna Yang

Tel:              +86 510 8525 9383 



Factory 2:



+86 151 5221 3005

Jiangsu Boli Bioproducts Co., Ltd.

93 Yangzhou Rd, Jiangyan Economic Development Zone,Taizhou City,Jiangsu 225500, China  

Jiangsu Boyang Bioproducts Co., Ltd.

30 Tongwang Road, Nantong Economic & Technological Development Zone,Nantong City,Jiangsu 226017, China

Boli USA Contact Information

PO Box 31096 Saint Louis,MO 63131 

Contact:David Singer 



Office:314 991 5191 

Mobile:314 503 0556 

Contact:Song Qian 


Contact:Ms.Edna Yang

Tel:+86 510 8525 9383 

Fax: +86 510 8522 1582 

Mobile:+86 151 5221 3005 



Address:93 Yangzhou Road,Jiangyan Economic Development Zone,Taizhou City,Jiangsu Province,China.

Boli China Contact Information