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Fungal Alpha-Amylase FA-280

An ideal Fungal Alpha-Amylase for alcohol production with the following benefits: ■ Full utilization of raw materials ■ Lower residual sugars at drop ■ Increased alcohol production ■ Lower residual starch in DDGS

Product Description

Fungal Alpha-Amylase FA-280 is made from non-genetic modified strains of Aspergillus oryzae var through deep fermentation and refining extraction technique. This enzyme can be used for starch liquefaction and also saccharification to produce maltose and glucose. It is an endoamylase which can rapidly hydrolyze the interior α-1, 4 glucosidic linkages of gelatinized starch, amylose and amylopectin solution and produce soluble dextrin and maltose and a small quantity of glucose.
FA-280 is applied to alcohol industries in addition to traditional glucoamylase to convert the starch fractions that can not be liquefied by bacterial alpha-amylase thereby increasing the efficiency of saccharification by glucoamylase.
Product Characteristics
Declared Enzyme:       Fungal Alpha-Amylase
Systematic Name: E.C., 1,4-α-D-Glucan glucanohydrolase
Activity: 28,000 U/ml (minimum)
Appearance: Sepia brown liquid
Product pH: 5.0 to 7.0
Specific gravity: 1.10 to 1.25 g/ml
Effect of pH
The optimum pH range of this product is 4.8~5.4 and starch can be effectively hydrolyzed at pH range 3.0~6.6. Typical effect of pH on enzyme activity is shown in Figure 1.
Effect of Temperature
Optimum temperature range is 45~55°C and effective temperature range is 30~65°C. Typical effect of temperature is shown in Figure 2.
This product may be inhibited by several heavy metal ions. Copper and lead ions are strong inhibitors. Inhibitive effects may also be achieved if the content of zinc, nickel and iron ions are high. 
This product is applied to saccharify corn, potato, tapioca, wheat, soyabean and starches from other resources. It can be hydrolyzed effectively if the content of starch on the dry substrate basis (DSB) is no more than 50%.
Dosage Guidelines
Use of FA-280 can be beneficial for alcohol production as follows: 
  • Full utilization of raw materials
  • Lower residual sugars at drop 
  • Increased alcohol production
  • Lower residual starch in DDGS 
FA-280 can be added together with Glucoamylase. Dosage of FA-280 depends on the actual situation such as starch, substrate concentration and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage rate is 0.005 to 0.01%w/w.
Packaging, Storage & Shelf-life
  • Standard packaging is 25kg, 30kg food-grace HDPE plastic drum, or 1125kg IBC totes.
  • Typical shelf-life is eighteen months provided this product is stored below 25°C (77℉) in its original packaging, sealed and unopened, protected from direct sunlight.
  • This enzyme has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, thus should be avoided. 
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with skins or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Material Safety Data Sheet.

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