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Product Description

AP-5000 is a high concentration Acid Protease produced from selected strains of Aspergillus niger through submerged fermentation, extraction and refining processes. It can catalyze hydrolysis of plant and animal proteins under acidic conditions .
AP-5000 has found its wide application in food industries, such as alcohol, fermentation and brewery, beer and wine production.
Product Characteristics
Declared Enzyme:
EC Number:
Pale brown powder
500,000 U/g
Moisture :
Effect of pH
AP-5000 can work at pH ranging from 2.0 to 6.0. For maximum activity, its optimal pH range is 2.5 to 4.0 at 40°C, with an optimum pH at 3.0 (see Figure 1).
Effect of Temperature
AP-5000 works best at temperatures from 30 to 50°C with 0.5% casein at pH around 3.0, and its optimum temperature is 40°C. It is unstable above 50°C and inactivated quickly above 60°C (see Figure 2).
Effect of Metal Ions
The enzyme activity of AP-5000 is activated by Mn2+, Ca2+ and Mg2+, whilst inhibited by Cu2+, Hg2+ and Al3+.
Use of AP-5000 can bring the following benefits for food industry:
  • Alcohol :  Helps to speed up fermentation and uplift yields of alcohol from starch materials.
  • Food Processing:  Enables an increased amino acid content of food, thus improve flavors and qualities of bread, cookies, cakes and sausages.
  • Beer Production:  Helps to decrease the diacetyl content, thus shorten brewing time.
AP-5000 is packaged in 20kg cardboard drums inner plastic bags or 20kg kraft bags. Customized packaging is available upon request.
Storage & Shelf-life
  • Avoid direct sunlight and rain during transportation.
  • The storage room should be kept clean, cool and dry, preferably with air-conditioner.
  • Typical shelf-life of this product is twelve months if it is stored below 25℃ in its original packaging, sealed and unopened, protected from direct sunlight. 
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.

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