Alpha-amylase HA-450LFG (Thermostable, Low pH)

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We are a technical leading enzyme manufacturer in China with an established reputation as a reliable supplier of quality enzyme products to our customers at home and abroad.

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Edna Yang

Tel: +86-510-8525-9383
Cell: +86-151-5221-3005
Email: edna.yang@bolibio.com

Jiangsu Boli Bioproducts Co.,Ltd.

Add: 93 Yangzhou Road, Jiangyan Economic Development Zone, Jiangsu 225500, China

Jiangsu Boyang Bioproducts Co., Ltd.

Add: 30 Tongwang Road, Nantong Economic Development Zone, Jiangsu 226010, China

Alpha-amylase HA-450LFG (Thermostable, Low pH)

With its robust alpha-amylase activity and superior thermal and pH stability, HA-450LFG has found its wide application in the production of alcohol.

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Enzymes for Alcohol & Brewing

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Product Description

Description

HA-450LFG is a food-grade alpha-amylase produced from Bacillus licheniformis through submerged fermentation and refining extraction processes. It is an endoamylase that can hydrolyze starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides, thus rapidly decrease the viscosity of gelatinous starch solutions. With its robust alpha-amylase activity and superior thermal and pH stability, HA-450LFG has found its wide application in the production of alcohol.

Product Characteristics

Declared Enzyme: Alpha-Amylase

Systematic Name: EC 3.2.1.1, 1,4-α-D-glucan glucanohydrolase

Activity: 200,000 U/ml (minimum)

Appearance: Sepia brown liquid

Product pH: 5.0 to 7.0

Specific gravity: 1.10 to 1.25 g/ ml

Effect of pH

HA-450LFG works at pH from 4.8 to 6.0, with its optimal pH from 5.0 to 5.9. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.

Effect of Temperature

For interval liquefaction, HA-450LFG works at temperatures from 80 to 100°C with its optimum temperature ranging from 83 to 86°C. It liquefies starch substrates promptly at 90 to 95ºC and maintains highly active at 100ºC. For consecutive liquefaction, HA-450LFG demonstrates its exceptional thermo-stability at high temperatures ranging from 105 to 110ºC and keeps on liquefying efficiently for 5 to 7 minutes.

Inhibitors

Copper, titanium, and cobalt ions are moderate inhibitors for HA-450LFG whilst aluminum, lead and zinc ions are strong inhibitors.

Dosage Guidelines

First, adjust pH to 5.0 to 5.9, and then add HA-450LFG. Heat the slurry for liquefaction at a temperature range from 83 to 86ºC for 90 to 150 minutes. The final DE value should be maintained at a range of 10 to 12.

Dosage of HA-450LFG is dependent on the actual application conditions including starch substrate, process, substrate concentration and other factors. To optimize the dosage rate, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting dosage rate is 0.10 ∼ 0.20kg/TDS.

Packaging, Storage & Shelf-life

HA-450LFG is packaged in 25kg, 30kg food-grade HDPE plastic drums or 1125kg IBC totes. Customized packaging is available upon request.
Typical shelf-life is six months if it is stored below 25°C in its original packaging, sealed and unopened, protected from direct sunlight.
Its shelf-life will be eighteen months if it is stored properly at 0∼10°C.
This enzyme has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, therefore should be avoided.

Safe Handling of Enzymes

Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.

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