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Product Description
PU-720 is typically used in combination with glucoamylase to produce high glucose syrup and high maltose syrup from liquefied starch.
Product Characteristics
| Declared Enzyme: | Pullulanase |
| Systematic Name: | EC 3.2.1.41, Pullulan 6 - glucanohydrolase |
| Appearance: | Sepia Brown Liquid |
| Activity: | 2,800 U/ml (minimum) |
| Product pH: | 4.0 to 4.5 |
| Specific gravity: | 1.10 to 1.20 g/ml |
Effect of pH
The effective pH range for PU-720 is from 4.0 to 5.0; its optimum pH ranges from 4.2 to 4.6. See Figure 1.
[Production of High-glucose Syrup]
Glucoamylase is capable of hydrolyzing alpha-1, 4 glycosidic bonds whilst slow in hydrolysis of alpha-1, 6 glycosidic bonds, which constitutes an obstacle in the production of high-glucose syrup. Use of pullulanase in combination with glucoamylase during the saccharification stage can increase the hydrolysis rate of starch, reduce the dosage of glucoamylase, shorten the reaction time, uplift the yield and improve the purity of glucose.
In the production of maltose, alpha-amylase is first used to liquefy the starch, then fungal alpha-amylase or beta-amylase shall be used to decompose starch, so the maltose content of the final product is about 50% to 60%, referred to as a high maltose syrup. Use of pullulanase in combination with alpha-amylase and beta-amylase can produce extra-high maltose syrup with maltose content above 80%.
In the production of beer, if the starch is not completely decomposed, the fermentable sugar content will be decreased, and the beer made from fermentation will not meet the requirements. The synergistic effect of pullulanase with alpha-amylase will enable pullulanase to completely decompose starch contained in the raw materials, reduce the wort content in the beta-limit dextrin, increase the amount of fermentable sugar, thus greatly improve the fermentation efficacy.
Adding pullulanase during saccharification in the production of MSG, alcohol, vinegar, and soy sauce can improve glucose content and shorten sacchrification time.
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Standard packaging is 25kg, 30kg food-grade HDPE drums or 1125 kg IBC totes.
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Typical shelf-life is six months if this product is stored below 25°C in its original packaging, sealed and unopened, protected from sunlight.
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Its shelf-life will be eighteen months if this product is stored properly at 0 ∼10°C.
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This product has been formulated for optimal stability.
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Prolonged storage and/or adverse conditions such as higher temperature may lead to a higher dosage requirement, therefore should be avoided.
Our pullulanase is a debranching enzyme specifically designed for the starch processing industry. Unlike conventional amylases, this pullulanase efficiently hydrolyzes α-1,6 glycosidic linkages in pullulan, amylopectin, and other branched polysaccharides. By incorporating this pullulanase into your liquefaction and saccharification processes, you achieve a higher dextrose equivalent (DE) and reduce residual oligosaccharides. The enzyme maintains over 90% activity at 60–65°C and pH 4.5–5.5, making it compatible with industrial mashing conditions.
Using pullulanase in combination with glucoamylase can increase glucose yield from starch by up to 5–8% compared to using glucoamylase alone. This pullulanase attacks branch points that other enzymes cannot access, releasing linear maltotriose and maltose chains for complete saccharification. For bioethanol production, adding pullulanase reduces viscosity and shortens fermentation time, as more fermentable sugars become available. Our pullulanase is produced from a selected Bacillus strain and is available in both liquid and powder formulations.
Quality control tests confirm that this pullulanase is free from protease and alpha-amylase side activities, ensuring predictable performance. It is Kosher and Halal certified, with a shelf life of 24 months when stored at 4–8°C. For continuous processes, the pullulanase shows excellent reusability when immobilized on chitosan beads. We provide technical support to optimize the dosage (typically 0.5–2.0 kg per ton of starch). Request a sample to see how this pullulanase can boost your starch conversion efficiency.
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