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Alpha-amylase HA-580LFG (Thermostable, Low pH)

Product Description

Description
HA-580LFG is a high concentrated food-grade alpha-amylase produced from Bacillus licheniformis through submerged fermentation and refining extraction processes. It is an endoamylase that can  hydrolyze starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to  produce soluble dextrins and oligosaccharides, thus decrease the viscosity of gelatinous starch  solutions rapidly. Use of HA-580LFG in the liquefaction of starch materials enables lower dosage level, less storage  and handling costs for the production of alcohol. 
 
Product Characteristic           
Declared Enzyme:       Alpha-Amylase
Systematic Name: EC 3.2.1.1, 1,4-α-D-glucan glucanohydrolase
Activity: 400,000 U/ml (minimum)
Appearance: Sepia brown liquid
Product pH: 5.0 to 7.0 S
pecific gravity: 1.10 to 1.25 g/ml

 

Effect of pH
HA-580LFG can work at pH from 4.8 to 6.0, with its optimal pH range from 5.0 to 5.9. The exact pH  optimum depends on the actual processing conditions, including temperature, substrate  concentration and processing time. 
 
Effect of Temperature 
For interval liquefaction, HA-580LFG works at temperatures from 80 to 100°C with it optimum  temperature range from 83 to 86°C. HA-580LFG can liquefy starch substrates promptly at 90 to  95ºC and maintain highly active at 100ºC.  For consecutive liquefaction, HA-580LFG demonstrates its exceptional thermo-stability at a  temperature range of 105 to 110ºC and keeps on liquefying efficiently for 5 to 7 minutes. 
 
Inhibitors
Copper, titanium, and cobalt ions are moderate inhibitors for HA-580LFG; aluminum, lead and zinc  ions are strong inhibitors for this enzyme. 
 
Dosage Guidelines
First adjust pH to 5.0 to 5.9, and then add HA-580LFG. Heat the slurry for liquefaction at a  temperature range from 83 to 86ºC for 90 to 150 minutes. Final DE value should be maintained at a  range from 10 to 12.  
Dosage of HA-580LFG depends on the actual conditions such as kind of starch, substrate concentration, processing time, temperature, pH, etc. To optimize the dosage, it is recommended  to conduct a number of liquefaction trials prior to routine use of this product. A good starting point  for dosage rate is 0.075 to 0.10KG/TDS.
 
Packaging, Storage & Shelf-life 
  • HA-580LFG is packaged in 25kg, 30kg food-grade HDPE plastic drums or 1125kg IBC totes.  Customized packaging is available upon request. 
  • Typical shelf-life is eighteen months if it is stored below 25°C in its original packaging, sealed and  unopened, protected from direct sunlight.  
  • This enzyme has been formulated for optimal stability. Prolonged storage and/or adverse  conditions such as higher temperatures may lead to a higher dosage requirement, therefore  should be avoided. 
 
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with skin or eyes,  promptly rinse with water for at least 15 minutes. For detailed handling information, please  refer to the Safety Data Sheet.
 

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