Alpha-amylase HT-200 (Thermostable)

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We are a technical leading enzyme manufacturer in China with an established reputation as a reliable supplier of quality enzyme products to our customers at home and abroad.

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Edna Yang

Tel: +86-510-8525-9383
Cell: +86-151-5221-3005
Email: edna.yang@bolibio.com

Jiangsu Boli Bioproducts Co.,Ltd.

Add: 93 Yangzhou Road, Jiangyan Economic Development Zone, Jiangsu 225500, China

Jiangsu Boyang Bioproducts Co., Ltd.

Add: 30 Tongwang Road, Nantong Economic Development Zone, Jiangsu 226010, China

Alpha-amylase HT-200 (Thermostable)

With its robust alpha-amylase activity and superior thermal and pH stability, HT-200 has found its wide application in liquefaction of starch in the production of starch sugar.

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Enzymes for Starch Sugar

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+86 510 8525 9383

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Product Description

Description

Heat-stable Alpha-Amylase HT-200 is produced from Bacillus licheniformis through submerged fermentation and refining extraction processes. As an endoamylase, HT-200 can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

With its robust alpha-amylase activity and superior thermal and pH stability, HT-200 has found its wide application in liquefaction of starch in the production of starch sugar.

Product Characteristics

Declared Enzyme:	Alpha-Amylase
Systematic Name:	EC 3.2.1.1, 1,4-α-D-glucan glucanohydrolase
Activity:	≥20,000 U/ml
Appearance:	Sepia brown liquid
Product pH:	5.0 to 7.0
Specific gravity:	1.10 to 1.25 g/ml

Effect of pH

HT-200 works at pH ranging from 5.0 to 7.0 with its optimal pH range from 5.4 to 6.0. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.

Effect of Temperature

For interval liquefaction, the optimum temperature of HT-200 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C. For consecutive liquefaction, HT-200 demonstrates exceptional thermal stability at a temperature range from 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.

Inhibitors

Copper, titanium, and cobalt ions are moderate inhibitors for HT-200 whilst aluminum, lead and zinc ions act as strong inhibitors.

Applications

HT-200 has been designed with the following benefits for the production of starch sugar:

Superior stability with low pH
Decrease viscosity of starch quickly
Eliminate the formation of unwanted color substances and non-fermentable sugars
Effective liquefaction of various starch materials, such as tapioca, corn, wheat, rice, etc.
Superior performance without adding calcium ion under majority processing conditions

Dosage Guidelines

For industrial application of one-time spray liquefaction, first prepare 30∼35% w/w starch slurry, adjust pH 5.4∼6.0, then add this product at a dosage rate of 0.8∼1.2 KG/TDS. Liquefy at 105∼107°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value.

In case the starch content is above 35%, recommended dosage rate is 1.2∼1.6KG/TDS.

Exact dosage of HT-200 depends on the actual situation such as starch substrate, liquefaction equipment, processing conditions, etc. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.

Packaging, Storage & Shelf-life

Standard packaging is 25kg, 30kg food-grade HDPE drums or 1125 kg IBC totes.

Typical shelf-life is six months if this product is stored below 25°C in its original packaging, sealed and unopened, protected from sunlight. Its shelf-life will be eighteen months if this product is stored properly at 0 ∼10°C. This product has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperature may lead to a higher dosage requirement, therefore should be avoided.

Safe Handling of Enzymes

Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.

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