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Alpha-amylase HA-440FG (Thermostable, Low pH)

Product Description

Heat-stable Alpha-Amylase HA-440FG is a food grade enzyme produced from selected strains of Bacillus licheniformis through submerged fermentation and refining extraction processes. It is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
With its robust Alpha-Amylase activity, superior thermal stability and low pH compatibility, HA-440FG has found its wide application in liquefaction of starch materials for alcohol production.
Product Characteristics
Declared Enzyme:
Systematic Name:
EC, 1,4-α-D-glucan glucanohydrolase
160,000 U/ml (minimum)
Sepia brown liquid
Product pH:
5.0 to 7.0
Specific gravity:
1.10 to 1.25 g/ml
Effect of pH
HA-440FG can work at pH ranging from 4.8 to 6.0 with its optimal pH from 5.4 to 5.9. Its exact optimum pH depends on actual processing conditions, including temperature, substrate concentration and reaction time.
Effect of Temperature
For interval liquefaction, HA-440FG works at temperatures ranging from 80 to 100°C with its optimum temperature range from 83 to 86°C. It liquefies starch substrates promptly at 90 to 95ºC and maintains highly active at 100ºC. For consecutive liquefaction, HA-440FG demonstrates its exceptional thermo-stability at a temperature range of 105 to 110ºC, keeps on liquefying efficiently   for 5 to 7 minutes.
Copper, titanium, and cobalt ions are moderate inhibitors for HA-440FG whilst aluminum, lead and zinc ions act as strong inhibitors.
Dosage Guidelines
First adjust pH to 5.4 to 5.9, and then add HA-440FG. After this enzyme is added, heat the slurry to liquefy at a temperature range from 83 to 86ºC for 90 to 150 minutes. The final DE value should be maintained at a range of 10 to 12.
Dosage of HA-440FG depends on the actual processing conditions such as starch substrate, application, process, substrate concentration and other factors. To optimize the dosage rate, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage rate is 0.18 to 0.22KG/TDS.
Packaging, Storage & Shelf-life 
  • HA-440FG is packaged in 25KG, 30KG food-grade HDPE plastic drums or 1125KG IBC totes. Customized packaging is available upon request.
  • Typical shelf-life is eighteen months if it is stored below 25°C in its original packaging, sealed and unopened, protected from direct sunlight.
  • This enzyme has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, therefore should be avoided.
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.

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