Glucoamylase HGA-101PT_Boli Bioproducts

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We are a technical leading enzyme manufacturer in China with an established reputation as a reliable supplier of quality enzyme products to our customers at home and abroad.

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Edna Yang

Tel: +86-510-8525-9383
Cell: +86-151-5221-3005
Email: edna.yang@bolibio.com

Jiangsu Boli Bioproducts Co.,Ltd.

Add: 93 Yangzhou Road, Jiangyan Economic Development Zone, Jiangsu 225500, China

Jiangsu Boyang Bioproducts Co., Ltd.

Add: 30 Tongwang Road, Nantong Economic Development Zone, Jiangsu 226010, China

Glucoamylase HGA-101PT

DESCRIPTION HGA-101PT is a Glucoamylase / Pullulanase blended enzyme. It contains robust Glucoamylase and starch debranching enzyme - Pullulanase. The Glucoamylase is produced from controlled fermentation of selected strains of Aspergillus niger , whilst the Pullulanase is produced from robust strains of Bacillus licheniformis. Glucoamylase can hydrolyze alpha-1,4 glucosidic linkages from the non-reduction end of starches to release glucose, whilst Pullulanase can specifically cleave the alpha-1, 6 glycosidic bond of the branching point of amylopectin, and cut out the entire branch structure to form amylose. Thus HGA-101PT has a higher conversion rate from starch to sugar.

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Enzymes for Starch Sugar

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+86 510 8525 9383

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Product Description

Description

HGA-101PT is a Glucoamylase / Pullulanase blended enzyme. It contains robust Glucoamylase and starch debranching enzyme - Pullulanase. The Glucoamylase is produced from controlled fermentation of selected strains of Aspergillus niger , whilst the Pullulanase is produced from robust strains of Bacillus licheniformis. Glucoamylase can hydrolyze alpha-1,4 glucosidic linkages from the non-reduction end of starches to release glucose, whilst Pullulanase can specifically cleave the alpha-1, 6 glycosidic bond of the branching point of amylopectin, and cut out the entire branch structure to form amylose. Thus HGA-101PT has a higher conversion rate from starch to sugar.

This compound enzyme product has been specially designed for the production of starch sugar.

Product Characteristics

Glucoamylase Activity:	100,000 U/ml (minimum)
Pullulanase Activity:	2,400 U/ml (minimum)
Appearance:	Yellow brown liquid
Product pH:	3.0 to 5.0
Specific Gravity:	1.10 to 1.25 g/ml

Effect of pH

HGA-101PT can work at pH range from 3.5 to 5.0. For maximum activity, the optimal pH range is 4.0 to 4.5, with an optimum pH at 4.2-4.5 (See Figure 1).

Effect of Temperature

Effective temperature range for HGA-101PT is 55 to 65°C, normal temperature range is 58 to 65°C , with an optimum temperature at 60°C. HGA-101PT can be inactivated at temperatures above 65°C. Normally, this product will be completely inactivated if the saccharified liquid is boiled at 80°C for 5 minutes (See Figure 2).

Applications

Use of HGA-101PT ensures the following benefits for starch sugar industry:

Greater conversion rate from starch to glucose
Maximized utilization of raw materials
Suitable for the production of glucose with DX > 97%
Capable to work with higher concentration substrate
Stable activity ensures stable dosage rate, no need to adjust dosage for each batch

Dosage Guidelines

Add this product into liquefied starches of substrates such as corn, wheat, barley, tapioca, rice, potatoes, etc. can convert the starches into glucose.

Recommended optimal temperature for industrial operations is 59 to 62°C, pH 4.1 to 4.5.

First, adjust pH; then add this product. Dosage rate depends on the type of raw materials and actual processing conditions. It is recommended to conduct several trials to determine the optimal dosage rate. A recommended dosage rate for HGA-101PT is 0.35 to 0.6 (typically 0.4 to 0.5kg / TDS. The saccharification should be kept for 32 to 48 hours depending on its actual processing requirements.

Packaging, Storage & Shelf-life

Standard packaging is 25kg, 30kg food-grade HDPE drums or 1125 kg IBC totes.
Customized packaging is available upon request.
Typical shelf-life is six months if it is stored below 25°C in its original packaging, sealed and unopened, protected from sunlight.
Its shelf-life will be eighteen months if this product is stored properly at 0 ∼10°C.
HGA-101PT has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperature may lead to a higher dosage requirement, therefore should be avoided.

Safe Handling of Enzymes

Inhalation of enzyme dust and mists should be avoided. In case of contact with skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.

Pullulanase PU-700

Fungal Alpha-Amylase FAA-280

Pullulanase PU-700

Fungal Alpha-Amylase FAA-280

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