HT-800 is a low pH tolerated Heat-stable Alpha-Amylase produced from selected strains of Bacillus licheniformis through submerged fermentation and refining extraction processes. HT-800 is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
As a robust Alpha-Amylase with superior thermal stability, HT-800 has found its wide application in the production of starch sugar.
Declared Enzyme: Alpha-Amylase
Systematic Name: EC 18.104.22.168, 1,4-α-D-glucan glucanohydrolase
Activity: 80,000 U/ml (Minimum)
Appearance: Sepia brown Liquid
Product pH: 5.0 to 7.0
Specific gravity: 1.10 to 1.25 g/ml
Effect of pH
HT-800 can work at pH ranging from 5.0 to 7.0 with its optimal pH range from 5.4 to 6.0. The exact pH optimum depends on the actual processing conditions, including temperature, substrate concentration and reaction time.
Effect of Temperature
For interval liquefaction process, the optimum temperature of HT-800 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C. For consecutive liquefaction process, HT-800 demonstrates exceptional thermal stability at temperatures from 105 to 110°C and keeps on liquefying efficiently for 5 to 7 minutes.
Concentration of Calcium
The presence of calcium increases the thermal stability of HT-800. This enzyme requires a very low calcium concentration of 20 to 50 ppm to ensure its enzyme stability.
Copper, titanium, and cobalt ions are moderate inhibitors for HT-800, whilst Aluminum, lead and zinc ions act as strong inhibitors.
Use of HT-800 can benefit the production of starch sugar as following:
1) Superior stability with low pH
2) Decrease viscosity of starch quickly
3) Eliminate undesirable color substances and non-fermentable sugars
4) Effective liquefaction of various starch materials, such as tapioca, corn, wheat, rice, etc.
5) Superior performance without adding calcium ion under majority processing conditions
For industrial application of one-time spray liquefaction, first prepare 30 to 35% w/w starch slurry, adjust pH 5.4 to 6.0, and then add this product at a dosage rate of 0.20 to 0.30 kg/TDS. Liquefy at 105 to 107°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value.
In case the starch content is above 35%, recommended dosage rate is 0.30 to 0.4kg/TDS.
Exact dosage rate of HT-800 depends on the actual processing conditions such as starch substrate, liquefaction equipment, pH, temperature, processing time, etc. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.
Packaging, Storage & Shelf-life
HT-800 is packaged in 25kg, 30kg food-grade HDPE plastic drums or 1125 kg IBC totes. Customized packaging is available upon request.
Typical shelf-life is six months if this product is stored below 25°C in its original packaging, sealed and unopened, protected from direct sunlight. Its shelf-life will be eighteen months if it is stored properly at 0∼10°C. HT-800 has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, and should be avoided.
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with skins or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.