Heat-stable Alpha-amylase HT-2400 is derived from strains of Bacillus licheniformis by submerged fermentation and refining extraction.
HT-2400 is an endoamylase, which rapidly decreases the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the α-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides. It can be applied without adding calcium ions during liquefaction process of production.
As a very robust alpha-amylase with low pH tolerance, HT-2400 is widely applicable to the production of starch sugar.
Declared Enzyme: Alpha-Amylase
Systematic Name: EC.220.127.116.11, α-1,4-glucan 4-glucanohydrolase
Activity: 240,000u/g (minimum)
Appearance: Beige-yellow powder
Moisture: 8% (Maximum)
Effect of pH
HT-2400 is active with pH range 5.0 to 7.0, the optimal pH is 5.4 to 6.0.
Effect of Temperature
For interval liquefaction, the optimum temperature of HT-2400 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C. For consecutive liquefaction, HT-2400 demonstrates exceptional thermal stability at temperatures up to 105 to 110°C , maintaining its stability for 5 to 7 minutes, continuing to liquefy rapidly, efficiently and completely.
Concentration of Calcium
The presence of calcium increases the thermal stability of HT-2400. A very low calcium concentration 20∼50 ppm is sufficient to ensure the enzyme stability.
Copper, titanium, and cobalt ions are moderate inhibitors for HT-2400 whereas aluminum, lead and zinc ions act as strong inhibitors.
In general, first adjust the pH to 5.4∼6.0 and then add calcium 20∼50 ppm before adding HT-2400. Calcium is not needed when tap water is used. After the enzyme is added, heat the slurry to liquefy. Dosage of HT-2400 depends on the actual situation such as starch, substrate concentration and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage rate is 0.03∼0.08kg/TDS.
HT-2400 is packaged in 25kg cardboard drums or 20kg kraft paper bags. Customized packaging is available upon request.
Storage & Shelf-life
This enzyme has been formulated for optimal stability. The typical shelf-life is six months if it is stored below 25°C in its original packaging, sealed and unopened, protected from direct sunlight. Its shelf-life will be eighteen months if it is stored properly at 0∼10°C. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, therefore should be avoided.
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with skins or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Material Safety Data Sheet.