Alpha-amylase HT-2400 (Thermostable)

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We are a technical leading enzyme manufacturer in China with an established reputation as a reliable supplier of quality enzyme products to our customers at home and abroad.

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Edna Yang

Tel: +86-510-8525-9383
Cell: +86-151-5221-3005
Email: edna.yang@bolibio.com

Jiangsu Boli Bioproducts Co.,Ltd.

Add: 93 Yangzhou Road, Jiangyan Economic Development Zone, Jiangsu 225500, China

Jiangsu Boyang Bioproducts Co., Ltd.

Add: 30 Tongwang Road, Nantong Economic Development Zone, Jiangsu 226010, China

Alpha-amylase HT-2400 (Thermostable)

Category：

Enzymes for Starch Sugar

Hotline：

+86 510 8525 9383

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Product Description

Description

Heat-stable Alpha-amylase HT-2400 is derived from strains of Bacillus licheniformis by submerged fermentation and refining extraction.

HT-2400 is an endoamylase, which rapidly decreases the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the α-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides. It can be applied without adding calcium ions during liquefaction process of production.

As a very robust alpha-amylase with low pH tolerance, HT-2400 is widely applicable to the production of starch sugar.

Product Characteristics

Declared Enzyme: Alpha-Amylase

Systematic Name: EC.3.2.1.1, α-1,4-glucan 4-glucanohydrolase

Activity: 240,000u/g (minimum)

Appearance: Beige-yellow powder

Moisture: 8% (Maximum)

Effect of pH

HT-2400 is active with pH range 5.0 to 7.0, the optimal pH is 5.4 to 6.0.

Effect of Temperature

For interval liquefaction, the optimum temperature of HT-2400 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C. For consecutive liquefaction, HT-2400 demonstrates exceptional thermal stability at temperatures up to 105 to 110°C , maintaining its stability for 5 to 7 minutes, continuing to liquefy rapidly, efficiently and completely.

Concentration of Calcium

The presence of calcium increases the thermal stability of HT-2400. A very low calcium concentration 20∼50 ppm is sufficient to ensure the enzyme stability.

Inhibitors

Copper, titanium, and cobalt ions are moderate inhibitors for HT-2400 whereas aluminum, lead and zinc ions act as strong inhibitors.

Dosage Guidelines

In general, first adjust the pH to 5.4∼6.0 and then add calcium 20∼50 ppm before adding HT-2400. Calcium is not needed when tap water is used. After the enzyme is added, heat the slurry to liquefy. Dosage of HT-2400 depends on the actual situation such as starch, substrate concentration and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage rate is 0.03∼0.08kg/TDS.

Packaging

HT-2400 is packaged in 25kg cardboard drums or 20kg kraft paper bags. Customized packaging is available upon request.

Storage & Shelf-life

This enzyme has been formulated for optimal stability. The typical shelf-life is six months if it is stored below 25°C in its original packaging, sealed and unopened, protected from direct sunlight. Its shelf-life will be eighteen months if it is stored properly at 0∼10°C. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, therefore should be avoided.

Safe Handling of Enzymes

Inhalation of enzyme dust and mists should be avoided. In case of contact with skins or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Material Safety Data Sheet.

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