Products

Contact us

+
  • undefined
  • undefined
  • undefined

Alpha-amylase HT-0400 (Thermostable)

DESCRIPTION Heat stable Alpha-Amylase HT-0400 is a powder enzyme preparation derived from Bacillus licheniformis through submerged fermentation and refining extraction process. It is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1, 4 glucosidic bonds to produce soluble dextrin and oligosaccharides.

Product Description

Description
Heat stable Alpha-Amylase HT-0400 is a powder enzyme preparation derived from Bacillus licheniformis through submerged fermentation and refining extraction process. It is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1, 4 glucosidic bonds to produce soluble dextrin and oligosaccharides.
HT-0400 is generally applicable to food industries including the production of starch sugar and brewing.
 
Product Characteristics
Declared Enzyme:       Alpha-Amylase
Systematic Name: EC 3.2.1.1, 1,4-α-D-glucan glucanohydrolase
Activity: 40,000 U/g (minimum)
Appearance: Light ivory powder
Moisture: 8% (Maximum)

 

Effect of pH
HT-0400 is active over a wide pH range, depending on the application. The effective pH range is 5.2~6.5. For maximum activity, the optimal pH for HT-0400 is 5.4 to 6.0. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.
 
Effect of Temperature
For interval liquefaction, the optimum temperature of HT-0400 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C. For consecutive liquefaction, HT-0400 demonstrates exceptional thermal stability at a temperature range from 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.
 
Inhibitors
Copper, titanium, and cobalt irons are moderate inhibitors for HT-0400 whereas aluminum, lead and zinc ions act as strong inhibitors.
 
Dosage Guidelines
In general, first adjust the pH to 5.4∼6.0 and then add calcium 20 to 50 ppm before adding  HT-0400. Calcium is not needed when tap water is used. After the enzyme is added, heat the  slurry to liquefy.Dosage of HT-0400 depends on the actual situation such as starch, substrate concentration and  other processing conditions. To optimize the dosage, it is recommended to conduct a number of  liquefaction trials prior to routine use of this product. A good starting point for dosage rate is  0.20 to 0.50kg/TDS. 
 
Packaging, Storage & Shelf-life
  • HT-0400 is packaged in 25kg cardboard drums or kraft paper bags. Customized packaging is  available upon request. 
  • Typical shelf-life is twenty-four months if this product is stored below 25°C in its original packaging,  sealed and unopened, protected from the sunlight.
  • Prolonged storage and/or adverse conditions such as higher  temperatures may lead to a higher dosage requirement. 
 
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes,  promptly rinse with water for at least 15 minutes. For detailed handling information, please  refer to the Safety Data Sheet.

Online message