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Glucoamylase ETHMEI GD

Product Description

Description
Glucoamylase ETHMEI GD is produced from strains of Aspergillus niger through submerged fermentation, extraction and refining processes. ETHMEI GD has a high conversion rate from starch substrates into fermentable sugars. It can hydrolyze α-D-1, 4 glucosidic bonds from the non-reducing end of starch one after another. It can also hydrolyze the α-D-1, 6 glucoside branch bonds of starch and cleaves the α-1, 3 bonds to release glucose.
With its robust glucoamylase activity, ETHMEI GD has found its wide application in the saccharification for the production of ethanol.
 
Product Characteristics
Declared Enzyme:       Glucoamylase
Systematic Name: EC 3.2.1.3, 1,4-alpha-D-glucan glucohydrolase
Appearance: Light to dark brown liquid
Activity: 300,000 U/ml (minimum)
Product pH: 3.0 to 5.0
Specific gravity: 1.10 to 1.20 g/ml
 
Effect of pH
ETHMEI GD can work at pH from 3.0 to 5.5. For maximum activity, its optimal pH range is 3.5 to 4.5. See Figure 1.
 
Effect of Temperature 
ETHMEI GD can work at temperatures from 30 to 65°C with its optimal temperature ranges from 55 to 60°C. See Figure 2.
                    
 
Applications
ETHMEI GD is used to saccharify the liquefied starches from various sources including corn, milo, barley, wheat, rice, potatoes and tapioca. The resultant glucose is then fermented by yeasts to yield ethanol.
For ground-grain processing plants using simultaneous saccharification fermentation (SSF), ETHMEI GD may be added directly to the fermenter. For ground-grain dry mill plants and for wet mill plants using a separate pre-saccharification step prior to fermentation, ETHMEI GD may be added to the cooling mash or liquefied starch when the temperature reaches 55 to 60°C. Temperatures above 65°C can result in loss of activity.
ETHMEI GD is designed to ensure the following benefits for ethanol production:
1)  Improved performance in pre-saccharification process
2)  Efficient starch/dextrin degradation
3)  Increased fermentation rate
4)  Less residual starch in distiller’s grains
5)  Increased ethanol yields
 
Dosage Guidelines
ETHMEI GD is generally added at a level of 0.35 to 0.45kg/TDS. The actual dosage rate depends on the raw materials, processing temperature, pH, reaction time, saccharification and fermentation process at individual ethanol plants.
 
Packaging, Storage & Shelf-life 
  • ETHMEI GD is packaged in 25kg, 30kg HDPE plastic drums or 1125kg IBC totes. Customized packaging is available upon request.
  • This enzyme has been formulated for optimal stability. Typical shelf-life is eighteen months provided it is stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight.
  • Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement.
 
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the appropriate Safety Data Sheet.

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