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Product Description

Acid Protease AP-6000 is produced from selected strains of Aspergillus niger through submerged fermentation, extraction and refining processes. It can catalyze hydrolysis of plant and animal proteins under acidic conditions . 
AP-6000 has found its wide application in food industries, such as alcohol, fermentation and brewery, beer and wine production. It can also be used in leather processing, wool coloration and waste film recovery. It can be further processed into medical-grade protease as anti-inflammatory agents, digestion supplements, or protease reagents.
Product Characteristics
Declared Enzyme:       
EC Number:
Pale Brown Powder
600,000 U/g (minimum)
Moisture :
8% (maximum)
Effect of pH
AP-6000 can work at pH ranging from 2.0 to 6.0. For maximum activity, its optimal pH range is 2.5 to 4.0 at 40°C, with an optimum pH at 3.0.
Effect of Temperature
The optimal temperature range for AP-6000 is 30 to 50°C with 0.5% casein at pH around 3.0, and its optimum temperature is 40°C. AP-6000 is unstable above 50°C and inactivated quickly above 60°C.
Effect of Metal Ions
The enzyme activity of AP-6000 is activated by Mn2+, Ca2+ and Mg2+, whilst inhibited by Cu2+, Hg2+ and Al3+.
 AP-6000 can speed up fermentation and uplift yields of alcohol from starch materials. 
Food Processing
 This enzyme can increase amino acid content of food, thus improve flavors and qualities of bread, cookies,
 cakes and sausages.
Beer Production
 AP-6000 can decrease the diacetyl content, thus shorten brewing time.
Animal Feed
 Use of this enzyme to hydrolyze proteins of animal feedstuff can help to maximize the utilization of feedstuff.
 Soften leather, enable easy dyeing, full hand-feel, improve leather and fur luster.
Packaging, Storage & Shelf-life
  • AP-6000 is packaged in 25kg water-proof kraft paper bags or cardboard drums with food grade plastic bags inside. Customized packaging is also available upon request.
  • Typical shelf-life is twelve months provided this product is stored at 0∼25°C in its original packaging, sealed and unopened, protected from direct sunlight.
  • Prolonged storage and/or adverse conditions such as higher temperatures may lead to higher dosage requirement, therefore should be avoided.
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Material Safety Data Sheet.

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