HA-360LFG is a heat-stable alpha-amylase produced from strains of Bacillus licheniformis through submerged fermentation and refining extraction processes. It is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
With its robust alpha-amylase activity and superior thermal and pH stability, HA-360LFG has found its wide application in the production of alcohol.
Declared Enzyme: Alpha-Amylase
Systematic Name: EC 22.214.171.124, 1,4-α-D-glucan glucanohydrolase
Activity: 180,000 U/ml (minimum)
Appearance: Sepia brown liquid Product
pH: 5.0 to 7.0
Specific gravity: 1.10 to 1.25 g/ ml
Effect of pH
HA-360LFG works at pH from 4.8 to 6.0 with its optimal pH from 5.0 to 5.9. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.
Effect of Temperature
For interval liquefaction, HA-360LFG works at temperatures from 80 to 100°C with its optimum temperature ranging from 83 to 86°C. It liquefies starch substrates promptly at 90 to 95ºC and maintains highly active at 100ºC. For consecutive liquefaction, HA-360LFG demonstrates its exceptional thermo-stability at high temperatures ranging from 105 to 110ºC and keeps on liquefying efficiently for 5 to 7 minutes.
Copper, titanium, and cobalt ions are moderate inhibitors for HA-360LFG whilst aluminum, lead and zinc ions are strong inhibitors.
First, adjust pH to 5.0 to 5.9, and then add HA-360LFG. Heat the slurry for liquefaction at a temperature range from 83 to 86ºC for 90 to 150 minutes. The final DE value should be maintained at a range of 10 to 12.
Dosage of HA-360LFG is dependent on the actual application conditions including starch substrate, process, substrate concentration and other factors. To optimize the dosage rate, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting dosage rate is 0.16 ∼ 0.20kg/TDS.
Packaging, Storage & Shelf-life
HA-360LFG is packaged in 25kg, 30kg food-grade HDPE plastic drums or 1125kg IBC totes. Customized packaging is available upon request.
Typical shelf-life is six months if it is stored below 25°C in its original packaging, sealed and unopened, protected from direct sunlight.
Its shelf-life will be eighteen months if it is stored properly at 0∼10°C.
This enzyme has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, therefore should be avoided.
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to Safety Data Sheet.