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Fungal Alpha-Amylase FAA-400

Product Description

Description
FAA-400 is a fungal alpha-amylase produced from strains of Aspergillus oryzae through submerged fermentation and refining extraction process. It can be used for both liquefaction and saccharification of starch materials to produce maltose and glucose. As an endoamylase, FAA-400can rapidly hydrolyze the interior α-1, 4 glucosidic linkages of gelatinized starch, amylose and amylopectin solution to produce soluble dextrin and maltose and a small quantity of glucose.
In the production of ethanol, FAA-400 can be used in combination of conventional glucoamylase to convert the starch fractions that cannot be effectively liquefied by bacterial alpha-amylase, thus improve the efficiency of saccharification by glucoamylase.
 
Product Characteristics
Declared Enzyme:       Fungal Alpha-Amylase
Systematic Name: EC 3.2.1.1,  1,4-α-D-Glucan glucanohydrolase
Activity: 28,000 U/ml (minimum)
Appearance: Sepia brown liquid
Product pH: 5.0 to 7.0
Specific gravity: ≤1.25 g/ml

 

Benefits
Use of FAA-400 provides the following benefits to ethanol producers:
  • Lower total residual sugars at drop
  • Increase ethanol production
  • Lower residual DP4+ material due to increased conversion
  • Lower residual starch in DDGS
  • Lower glycerol production due to a more proportional glucose/maltose profilereducing osmotic stress
 
Effect of pH
The optimum pH range of FAA-400 is 4.8 to 5.4 and starch can be effectively hydrolyzed at pH range 3.0 to 6.6. Typical effect of pH on enzyme activity is shown in Figure 1.
 
Effect of Temperature 
Optimum temperature ranges from 45 to 55°C and effective temperature range is from 30 to 65°C. Typical effect of Temperature is  shown in Figure 2.
Inactivation
Inactivation can be achieved by raising the temperature to 90 to 100°C and maintaining for approximately 5 to 10 minutes. If the content of starch is higher, this product can be inactivated by keeping the temperature at 80°C for about 30 minutes, or 70°C for about 60 minutes. If inactivation by high temperature is not desirable, another option of inactivation is to decrease the pH below 4.0. Inactivation can also be realized by adjusting pH and temperature at the same time.
 
Inhibitors
FAA-400 can be inhibited by several heavy metal ions. Copper and lead ions are strong inhibitors. Inhibitive effects may also be achieved if the content of zinc, nickel and iron ions are high. 
 
Dosage Guidelines
FAA-400 is used to saccharify corn, potato, tapioca, wheat, soyabean and starches from other  resources. It can be hydrolyzed effectively if the content of starch on the dry substrate basis (DSB)  is less than 50%. FAA-400 can be added in combination with Glucoamylase. Dosage of FAA-400 depends on the  actual situation such as starch, substrate concentration and other processing conditions. To  optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine  use of this product. A good starting point for dosage rate is 0.005 to 0.01%w/w. 
 
Packaging
FAA-400 is packed in 25kg, 30kg HDPE plastic drums or 1125kg IBC totes. Customized packing is  also available upon demand.  
 
Storage & Shelf-life 
  • Typical shelf-life is eighteen months provided it is stored below 25°C in its original packaging, sealed and  unopened, protected from direct sunlight.
  • Prolonged storage and/or adverse conditions such as higher temperatures  may lead to a higher dosage requirement, therefore should be avoided. 
 
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with skins or eyes,  promptly rinse with water for at least 15 minutes. For detailed handling information, please refer  to the Safety Data Sheet.

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