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Product Description

Acid Protease AP-150 is produced from selected strains of Aspergillus niger through submerged fermentation, extraction and refining processes. It can catalyze the hydrolysis of proteins under acidic conditions. AP-150 is applicable to hydrolysis of proteins with acidic medium.
As a robust acid protease, AP-150 has found its wide application in the production of ethanol.
Product Characteristics
Declared Enzyme:      
Acid Protease
Systematic Name:
150,000 U/ml (minimum)
Black brown Liquid
Product pH:
2.5 to 5.0
Specific Gravity:
≤1.25 g/ml
Effect of pH
AP-150 can work at pH range from 2.0 to 6.0 with its optimal pH from 2.5 to 3.5.
Effect of Temperature
AP-150 can work at temperatures from30 to 65°C (86 to 149℉) with its optimal temperature from 40 to 50°C (104 to 122℉).
Effect of Metal Ions
AP-150 is activated by Mn2+, Ca2+ and Mg2+, and inhibited by Cu2+, Hg2+ and Al3+.
Use of AP-150 in fermentation during ethanol production offers the following benefits:
1)  Enable raw material particles to be dissolved quickly
AP-150 can hydrolyze the proteins of the raw materials, thus destroy the interstitial cell wall structure of starch granules so as to release the starch wrapped in the granules, which is conducive to saccharification.
2)  Promote the growth of yeast
Free amino nitrogen (FAN) is the most effective nitrogen source for yeast. Adding AP-150 during alcoholic fermentation can effectively hydrolyze proteins and peptides in raw materials so as to generate FAN that yeasts can absorb, thus promote the growth of yeasts, increase the quantities of yeasts in the fermented mash, and shorten the fermentation cycle.
3)  Beneficial to high concentration fermentation
In high concentration fermentation of alcohol, it is mainly nutritional deficiencies rather than the accumulation of alcohol that leads to the decrease of fermentation activity. Free amino acids (FAN) can not only shorten the fermentation cycle but also increase the output of alcohol, improve ethanol tolerance and survival rate of yeasts. Therefore, AP-150 is the best nutrient for yeast growth and accelerator for fermentation.
Application Guidelines
In intermittent fermentation, it should be added at one time when the fermentation tank is filled with one third materials. In continuous fermentation, it should be added consecutively depending on the amount of saccharification mash fed at the inlet of fermentation tank.
Dosage Recommendation
Recommended dosage of AP-150 is 0.001 to 0.01% w/w (dry solids basis). Actual dosage should be depending on the raw materials and process of production.
AP-150 is packaged in 25kg, 30kg plastic drums or 1125kg IBC totes. Customized packaging is available upon request.
Storage & Shelf Life                                                                                                                                       
  • Typical shelf-life is twelve months if this product is stored at 0∼25°C (32∼77℉) in its original packaging, sealed and unopened, protected from direct sunlight.
  • Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, thus should be avoided.
Safe Handling of Enzymes
Inhalation of enzyme dust and mists should be avoided. In case of contact with skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.

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