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Glucoamylase PUREMEI PG

Product Description

Description
Glucoamylase PUREMEI PG is made from NON-GMO strains ofAspergillus nigerthroughsubmerged fermentation, extraction and refining processes.PUREMEI PG has a high conversion rate of starchy substrates into fermentable sugars. Itcanhydrolyze α-D-1, 4 glucosidic bonds from the non-reducing end of starch one after another. It can also hydrolyze the α-D-1, 6 glucoside branch bonds of starch and cleaves the α-1, 3 bonds to release glucose.
PUREMEI PG is designed with a robust glucoamylase activity for saccharification in fuel ethanol production.Use of PUREMEI PG provides higher ethanol yields, faster fermentation and less starch residues.
 
Product Characteristics
Declared Enzyme:       Glucoamylase
Systematic Name: EC 3.2.1.3, 1,4-alpha-D-glucan glucohydrolase
Appearance: Light to dark brown liquid
Activity: 130,000 U/ml (minimum)
Product pH: 3.0 to 5.0
Specific gravity: 1.10 to 1.20 g/ml

 

Effect of pH
The optimal pH for PUREMEI PG ranges from 3.5 to 4.5. See Figure 1.
 
Effect of Temperature 
The effective temperature for PUREMEI PG is from 30 to 65°C, and its optimal temperature range is from 55 to 60°C.
See Figure 2.
Figure 1: 
Figure 2: 
Substrate: 4%(w/v) Soluble Starch
Temperature: 60℃
Time: 60 min
Substrate: 4%(w/v) Soluble Starch
pH: 4.2
Time: 60 min
 
Applications
PUREMEI PG is used to saccharify liquified mash from various sources including corn, milo, barley, wheat, rice, potatoes and tapioca. The resultant glucose product is fermented by yeast or another appropriate microorganism to yield ethanol.
For ground-grain processing plants using simultaneous saccharification fermentation (SSF), PUREMEI PG may be added directly to the fermenter. For ground-grain dry mill plants and for wet mill plants using a separate pre-saccharification step prior to fermentation, PUREMEI PG may be added to the cooling mash or liquefied starch when the temperature reaches 55 to 60°C.Temperatures above 65°Ccan result in loss ofactivity rapidly.
 
Dosage Guidelines
PUREMEI PG is generally added at a level of 0.065 to 0.085% w/w (dry solids basis). The optimal dose depends on the actual processing conditions including raw materials, temperature, reaction time, pH and the desired results in individual ethanol plants.

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