Catalase CA-540 is an oxidoreductase derived from submerged fermentation of microorganism through extraction and refining process. It decomposes hydrogen-peroxide into water and oxygen in a highly efficient way without any side effects on textiles and dyestuffs.
Catalase CA-540 is applied to eliminate hydrogen peroxide residues originating from the bleaching process of textile fabrics and to improve dyeing quality with reduced processing time and less effluents. It is also used to prevent skin irritation that might be caused due to the presence of hydrogen peroxide residues in textile fabrics after bleaching and/or sterilizing at hotels, hospitals and laundries. As an enzyme concentration, Catalase CA-540 can be used directly or after dilution. It is more convenient for transportation and storage.
Declared Enzyme: Catalase
Systemic Name: EC 22.214.171.124 , Hydrogen-peroxide oxidoreductase
Activity: 200,000 U/ml (minimum)
Appearance: Light to dark brown liquid
Product pH: 5.0 to 8.0
Specific gravity: 1.10 to 1.25 g/ml
Application of catalase CA-540 to the elimination of hydrogen peroxide only requires cold bath for once. Inactivation and washing is not needed before dyeing. The elimination of hydrogen peroxide can be conducted in the same bath of dyeing without washing, resulting in considerable savings of rinsing water ( at least saved by 3/4 in average).
CA-540’s characteristic of decomposing hydrogen peroxide in a highly efficient way results in big saving of washing time, which is roughly half of the preparation time before dyeing process.
Elimination of hydrogen peroxide with CA-540 does not need heating and the process flow is comparatively short, thus allowing less electricity consumption.
CA-540 does not have any effects on dyeing performance of vat dyes. Hydrogen peroxide is decomposed into water and oxygen, which do not have any effects on dyes either.
Hydrogen peroxide is decomposed into water and oxygen without hazardous effluents, allowing considerable savings of wastewater treatment costs.
Effect of pH and Temperature
CA-540 is active at a wide pH range from 6 to 10 at temperatures from 30 to 60 ºC. Its optimal pH is 7.0 at the optimal temperature of 40 ºC.
Effect of Substrate Concentration
The reaction speed of CA-540 has a positive correlation with the concentration of substrates when the concentration of hydrogen peroxide is less than 1000ppm. However, the reaction speed decreases when the concentration of hydrogen peroxide is excessive high.
Take batch-wise processing for instance:
Step 1. Discharge bleaching solutions completely;
Step 2. Wash with cold water for once, or add water directly to the required bath of dyeing
Step 3. Adjust pH to 6 to 10 (recommended to use glacial acetic acid ) and control temperature below 55 ºC;
Step 4. Add CA-540 at a dosage rate of 0.025 to 0.05ml enzyme per liter of water (dosage can be more in case of limited processing time is allowed due to consecutive operational equipment);
Step 5. Keep the reaction for 10 to 20 minutes, then examine ( with Merck reagent or any other common peroxide reagent) whether the hydrogen peroxide is eliminated completely or not. If not, keep the reaction for another 5 to 10 minutes andthe decomposition shall complete thoroughly;
Step 6. Continue to dyeing with the same bath, or wash the bleached or sterilized fabrics with water and take it out.
The exact dosage and application method should be adjusted depending on the actual situation.